Salivary Cortisol,MS is a specialized test that measures cortisol levels in saliva using mass spectrometry technology. This non-invasive method provides a convenient alternative to blood testing and is particularly useful for measuring free (unbound) cortisol levels, which represent the biologically active form of the hormone. Salivary cortisol testing is valuable for evaluating the diurnal rhythm of cortisol production, as it can be collected multiple times throughout the day without the stress of repeated blood draws. This test is particularly useful for diagnosing Cushing's syndrome, adrenal insufficiency, and other disorders of cortisol metabolism. The salivary method also helps avoid the stress response that can occur with blood collection, providing more accurate baseline cortisol measurements.