Total Glutathione is a comprehensive test that measures the body's primary antioxidant and cellular defense molecule. Glutathione exists in three forms: reduced glutathione (GSH), oxidized glutathione disulfide (GSSG), and protein-bound glutathione (GSSP). As the most abundant intracellular antioxidant, glutathione plays a crucial role in protecting cells from oxidative damage, detoxifying harmful substances, and maintaining cellular redox balance. Decreased glutathione levels have been associated with various disease states including Parkinson's disease, HIV infection, liver disease, renal failure, and preeclampsia. This test is primarily used for research purposes and to evaluate oxidative stress status in patients with chronic diseases. Understanding glutathione levels can help assess cellular antioxidant capacity and may guide therapeutic interventions aimed at improving oxidative stress management.