SARS-CoV-2 Antibody Profile, Nucleocapsid and Spike is a comprehensive test that measures antibodies against two different viral proteins to distinguish between natural infection and vaccination. This test detects antibodies against the nucleocapsid protein (indicating natural infection) and the spike protein (which can result from either infection or vaccination). The ability to differentiate between these antibody types is crucial for evaluating patients with a history of COVID-19 vaccination who may have also experienced natural infection. This distinction is particularly important for diagnosing Multisystem Inflammatory Syndrome in children (MIS-C) or adults (MIS-A), where determining the source of antibodies can guide treatment decisions. The test helps healthcare providers understand a patient's complete COVID-19 exposure history and assess their immune response to both natural infection and vaccination.